2009
Videos
Dumbbell-shaped, dividing chloroplasts and movement - Wright et al., (2009):American Journal of Botany 96: 865-876. Supplementray data Appendix S1
Chloroplast streaming in "mature perforation" stage leaves. Wright et al., (2009). American Journal of Botany 96: 865-876. Supplementray data Appendix S2
Late-stage mitochondria streaming. Wright et al., (2009). American Journal of Botany 96: 865-876. Supplementray data Appendix S3
Cessation of mitochondria streaming prior PM collapse. Wright et al., (2009). American Journal of Botany 96: 865-876. Supplementray data Appendix S4
Lace plant leaf cells undergoing PCD - Wright et al., (2009):American Journal of Botany 96: 865-876. Supplementray data Appendix S5
Transvacuolar strands - Wright et al., (2009):American Journal of Botany 96: 865-876. Supplementray data Appendix S6
Brownian motion of chlorophyll-containing material in vacuole (aquarium–grown). Wright et al., (2009):American Journal of Botany 96: 865-876. Supplementray data Appendix S7
Cytoplasmic streaming over a gradient of PCD stages - Wright et al., (2009):American Journal of Botany 96: 865-876. Supplementray data Appendix S8
2010
Mitochondria and chloroplasts in healthy cell- Lord and Gunawardena (2010) Planta (233:407-421 On line resource 3a). Actively streaming mitochondria in lace plant protoplast (control). CMXRos stained mitochondria (red) and cholorphyll autofluorescence (green).
Mitochondria and chloroplasts in dying cell - Lord and Gunawardena (2010) Planta (233:407-421 On line resource 3b). Dying Mitoc Protoplast HS at 55°C for 20 minutes followed by a 30 minute rest period at room temperature. Note there is no actively streaming mitochondria within the HS challenged cell, when compared to the control sample (Online resource 3a). Also note the decrease in chloroplast abundance within the cell.
2011
Lord, C, Wertman, J., Lane, S. and Gunawardena, A.H.L.A.N. (2011). Do mitochondria play a role in remodelling lace plant leaves through programmed cell death?” BMC Plant Biology 11:102
http://www.biomedcentral.com/1471-2229/11/102
CMXRos stained NPCD cell, highlighting stage M1 mitochondrial dynamics. Note individual mitochondria actively streaming within the cytosol.
CMXRos stained EPCD cell, highlighting stage M2 mitochondrial dynamics. Note the aggregation of mitochondria along with several individual mitochondria, all of which appear to be moving.
CMXRos stained EPCD cell and DIC overlay. Video highlights the transition from stage M2 to stage M3 mitochondria. Note mitochondrial aggregate moving towards the nucleus, followed by cessation of movement . Video is 15X normal speed.
CMXRos stained LPCD cell, highlighting stage M3 mitochondrial dynamics. Note the absence of movement of the mitochondrial aggregate.
CMXRos stained LPCD cell, aimed at highlighting stage M4 mitochondrial dynamics. Note the lack of mitochondrial staining by CMXRos possibly due to complete loss of ΔΨ m.
NPCD stage cells showing several transvacuolar strands, and highlighting the close association and possible movement of mitochondria and chloroplasts along them. Video 20X normal speed
EPCD stage cells showing increased transvacuolar strands activity and highlighting the close association and possible movement of mitochondria and chloroplasts along them. Note the trajectory of most strands and organelles towards the nucleus. Video 20X normal speed
LPCD stage cells showing a decrease in the number of transvacuolar strands and absence of organelles. Note that although mitochondrial streaming in often ceased at this point, slight cytoplasmic streaming can be visualized in mesophyll cells below the point of focus. Video 20X normal speed.
CsA treated leaf subsequently stained with CMXROS, depicting a single cell that corresponds with an NPCD window stage cell. Note, individual mitochondria that are rapidly moving within the cytosol.
CsA treated leaf subsequently stained with CMXROS, depicting a single cell that corresponds with an EPCD window stage cell. Note, individual mitochondria that are rapidly moving in the cytosol.
CsA treated leaf subsequently stained with CMXROS, depicting a single cell that corresponds with an LPCD window stage cell. Note, many individual mitochondria, and several small aggregates that are rapidly moving .
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